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2 edition of Quantitative HPLC determination of plant lignans and isoflavones in cereal grains found in the catalog.

Quantitative HPLC determination of plant lignans and isoflavones in cereal grains

Huei-Fen Hsia

Quantitative HPLC determination of plant lignans and isoflavones in cereal grains

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  • 11 Currently reading

Published .
Written in English

    Subjects:
  • Grain -- Composition.,
  • Lignans.,
  • Isoflavones.

  • Edition Notes

    Other titlesQuantitative high-performance liquid chromatographic determination of plant lignans and isoflavones in cereal grains.
    Statementby Huei-Fen Hsia.
    The Physical Object
    Paginationviii, 36 leaves, bound :
    Number of Pages36
    ID Numbers
    Open LibraryOL19224781M

    A Comparative Study on Proximate Composition, Phytochemical Screening, Antioxidant and Antimicrobial Activities of Linum usitatisimum L. (flaxseeds) Tawheed Amin1* and Monika Thakur2 1Department of Food Technology, Islamic University of Science and Technology, Awantipora, Jammu & Kashmir 22 . The Flax Council of Canada is a national organization which promotes Canadian flax and flax products for nutritional and industrial uses in domestic and international markets. Qualitative and quantitative analysis of the major phenolic compounds as antioxidants in barley and flaxseed hulls using HPLC/MS/MS – Flax Council Of Canada. in almost all plant, microorganism, fungi and animal tissue. Flavonoids constitute a large group of naturally occurring plant phenolic compounds. Flavonoids, including flavones, flavonol, isoflavones, flavonones, and chalcones occurs in all type of plant tissue. Flavones and flavonol are found in almost every plant, especially in the leave and. plant densities of , plants/ha plants/ha, respectively. Significant differencesin soybean seed isoflavones (daidzein, genistein and glycitein) contents have been observed between plants grown in the two different plant densities. The mean daidzein content was µgg−1 in.

    The aim of this study was to investigate the effect of Improved Extrusion Cooking Treatment (IECT) on the phenolics and its bioaccessibility in cereals, represented by brown rice, wheat, and oat. Data showed that total phenolic content and total antioxidant activity in free form were significantly decreased, while the bound form was increased after IECT. After IECT, the total free phenolic Cited by:


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Quantitative HPLC determination of plant lignans and isoflavones in cereal grains by Huei-Fen Hsia Download PDF EPUB FB2

Methylated isoflavones found in clover, which may be used in red clover dietary supplements, and they are metabolized in the body to daidzein and genistein, respectively. Lignans are found in flax seeds, whole wheat flour, tea, some fruits, and other cereal grains. Lignans include matairesinol.

Turk J Pharm Sci 11(2),Original article Quantitative Determination of Isoflavones by HPLC-UV Method and Antioxidant Activity of Trifolium longidentatum Nurgiin KUCÜKBOYACI', Özlem. Quantitative determination of lignans and isoflavonoids in plasma of omnivorous and vegetarian women by isotope dilution gas chromatography-mass spectrometry.

Adlercreutz H(1), Fotsis T, Lampe J, Wähälä K, Mäkelä T, Brunow G, Hase T. Author information: (1)Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, FinlandCited by: A reversed phase HPLC method was established for quantitative determination of lignans including schizandrin (1), gomisin A (2), deoxyschizandrin (3), gomisin N (4), and wuweizisu C (5) from.

Sun J M, Ding A L, Chang R Z. Genetic analysis on isoflavone content in soybean seeds. Agricultural Sciences in China, 1, Sun J M, Ding A L, Dong H R. High performance liquid chromatographic determination of isoflavone content in soy- Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds 77 Cited by:   An increasing number of nutritional supplements contain phytoestrogens, in particular isoflavones, which potentially alleviate climacteric complaints.

Intention of the present study was the qualitative and quantitative determination of isoflavones in soy based nutritional supplements in order to compare the actual content with the labeling of these by: Lignans constitute a group of important plant phenols that are structurally characterized by the coupling of two phenylpropanoid units by a bond between the β-positions in the propane side chains ().According to recent nomenclature recommendations, the units are treated as propylbenzene units, giving the positions 8 and 8′ to these linked carbon by:   Abstract.

Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF).Cited by: 5.

A new and simple analytical method using HPLC with fluorescence detection was developed for the simultaneous determination of four lignans (phyllanthin, hypophyllanthin, phyltetralin and niranthin) in Phyllanthus niruri L. plant samples.

Optimal separation was achieved with an isocratic mobile phase consisting of acetonitrile-water ( v/v).Cited by: ), and direct comparison (TLC, HPLC) with authentic sample of quercetin. Supercritical Extraction A sample of dried plant material was used for extraction in a supercritical extraction (SE) pilot plant (Cassel et al., ).

The solvent used was % CO2 with a flow rate of. Plant lignans are diphenolic compounds ingested with whole grains and seeds and converted to enterolignans by the colonic microbiota. In the present study, we investigated absorption and metabolism of plant lignans and enterolignans in vivo after consumption of cereal-based diets.

Six pigs fitted with catheters in the mesenteric artery and portal vein and with a flow probe attached to the Cited by: 9. Isoflavones are one of the major groups of phytoestrogens found in the human diet and legume is a good source of isoflavones and lignans, molecules with antioxidant properties that, among other effects, might help to fight and prevent several pathologies (Cederroth & Nef, )5.

Isoflavones are a class of plant. A new analytical method, based on liquid chromatography (LC) with UV-diode array detection, for the simultaneous determination of daidzein, genistein, and glycitein and their 7-O- β -D-glucopyranoside (daidzin, genistin, and glycitin, resp.) has been successfully developed.

All the calibration curves showed good linearity within the concentration range &#x;2&#x;μg/ by: 3. Comparison between the number of 2D-PAGE protein spots of the storage protein subunits and HPLC area of twelve isoflavones was also evaluated.

The analysis of proteins and isoflavones from the wild genotype and the three cultivated genotypes suggested possible interactions between proteins and : Savithiry S. Natarajan, Devanand L. Luthria, Qijian Song, Perry Cregan. Twenty-four plant lignans were analyzed by high-performance liquid chromatography−tandem mass spectrometry in bran extracts of 16 cereal species, in four nut species, and in two oilseed species (sesame seeds and linseeds).

Eighteen of these were lignans previously unidentified in these species, and of these, 16 were identified in the analyzed by:   HPLC–UV method for detection of hypophyllanthin and phyllanthin from herbal hepatoprotective ef, CAS, Google Scholar; 17 Murugaiyah V, Chan KL.

Determination of four lignans in Phyllanthus niruri L. by a simple high-performance liquid chromatography method with fluorescence detection. Chromatogr. A (1–2), – Cited by: 1. lignans. The isolavones considered in this report are daidzein, genistein, O-desmethylangolensin (ODMA), and equol.

The lignans considered in this report are enterodiol and enterolactone. Plant sources of isolavones include legumes, with the largest contribution coming from soy-based foods. Since soy lour and soy protein isolates. the plant material being analyzed and of the limitations of the analytical method being used.

The structure of lignins is discussed extensively by Sarkanen and Ludwig. 1 and by Adler,2 and various lignin determination procedures are discussed in detail by Browning.3 (As is common in ligninFile Size: KB.

I.Šimonová et al.: HPLC Determination of Inulin in Plant Materials Acta Chimica Slovaca, Vol.3, No.2,- Materials and Methods Preparation of lactic acid fermented juices The fresh cabbage was purchased in a local market in Slovakia. From the cabbage, the outer leaves were removed and the cabbage was chopped to small slices.

Flavonoids are the most abundant polyphenols in our daily diets ().They can be divided into different classes based on the chemical structure of heterocyclic ring as flavones, flavanones, flavonols, etc ().High concentration of flavones are found in dietary plants, like herbs (parsley mg/ g of edible portion), fruits (blueberries mg/ g of edible portion), and vegetables (mustard.

New HPLC-chemometric approaches were proposed for the simultaneous chromatographic quantification of daidzein, genistein, formononetin, and biochanin A in the samples consisting of the aerial parts of Trifolium lucanicum Gasp.

(Leguminosae). Partial least squares and principal component regression algorithms were applied to the multiple chromatographic data set obtained by measuring at Cited by: 2.

Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 by: that include isoflavones, lignans, coumestans, resorcylic acid lactones, and mycotoxins, 23–25 Significant in vitro estrogenic responses were seen with two isoflavones, daidzein and genistein, and their respective precursors, formononetin and biochanin A Exertion of.

Phytate Æ Quantitative trait loci Æ Rice Introduction Human nutritionists regard phytate (phytic acid or phytin) as a major anti-nutrient present in le-gume seeds and cereal grains, reducing the bio-availability of dietary Zn and non-heme Fe.

However, reducing the level of phytate in plant foods is still under debate. While breeding for lowCited by:   Generally, HPLC with a methanol and water elution gradient is used for isoflavone quantification, but capillary zone electrophoresis (CZE) has been used more recently to separate several flavonoids in plant extracts.

We present the results of CZE analysis of isoflavones in soybean by: Many methods reported in literature for the analysis of soy isoflavones are based on high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). They have been developed to quantify several isoflavones simultaneously, however, involve laborious processes of sample preparation, separation, detection.

Dietary guidelines universally advise adherence to plant-based diets. Plant-based foods confer considerable health benefits, partly attributable to their abundant micronutrient (e.g., polyphenol) content. Interest in polyphenols is largely focused on the contribution of their antioxidant activity to the prevention of various disorders, including cardiovascular disease and cancer.

Polyphenols Cited by: The present paper aims at reviewing and commenting on the analytical methods applied to antioxidant and antioxidant capacity assessment in plant-derived products.

Aspects related to oxidative stress, reactive oxidative species’ influence on key biomolecules, and antioxidant benefits and modalities of action are discussed. Also, the oxidant-antioxidant balance is critically by: ABSTRACT. Studies were carried out on the optimization of the extraction procedures for isoflavones determination in soybean by HPLC.

Isoflavones were extracted from mg samples of full fat soybean flour "Kinako" (milled toasted grains). Soybean seed isoflavones include three aglycones (daidzein, genistein and glycitein), their glycosides, and their corresponding acetyl and malonyl derivatives [21].

Soybean isoflavones have a positive impact on human health, including prevention of chronic diseases, includ- ing cancer, heart disease, osteoporosis, and menopausal. Abstract A high performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection was developed for determining secoisolariciresinol diglucoside (SDG) in extracts of flaxseed from different locations in Gansu province, China.

The optimized method was achieved for the separation and detection of selected constituents using acetonitrile‐1% aqueous Cited by: antioxidant, determination (qualitative and quantitative) of some flavonol was performed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).

A- TLC Qualitative estimation of flavonol (Quercetin, Kampferol, Rutin and Myricetin) in dried leaves extract of Iraqi Eruca. c Indian Academy of Sciences RESEARCH ARTICLE Quantitative trait loci analysis of individual and total isoflavone contents in soybean seeds HAI JUN ZHANG1,2, JING WEN LI 1, YA JING LIU, WEN ZHU JIANG1, XING LIN DU 1,LINLI, XIAO WEI LI, LIAN TAI SU 1,QINGYUWANG∗ and YING WANG1∗ 1College of Plant Science, Jilin University, ChangchunJinlin, People’s Republic of China.

of the plant material was taken for quantitative estimation. Determination of total phenols by spectrophotometric method: The fat free sample was boiled with 50 ml of ether for the extraction of the phenolic component for 15 min. 5 ml of the extract was taken into a 50 ml flask, then 10 ml of distilled water was added.

2 mlFile Size: KB. Visit to get more information about this book, to buy it in print, or to download it as a free PDF. Determination of rutin and quercetin in buckwheat farina and bran by HPLC Chromatography conditions HPLC analysis was performed according to the following conditions: Dalian Elite YPERSIL 5 µm x mm C18 column (China), methonal phosphoric acid water solution () as the mobile phase, with a detection wavelength of by:   Simultaneous determination of nine lignans from Schisandra chinensis extract using ultra‐performance liquid chromatography with tandem mass spectrometry in rat plasma, urine, and gastrointestinal tract samples: Application to the pharmacokinetic study of Schisandra chinensis.

Uses plant Gogo (bark) Entada phaseoloides as control. The positive result shows a honeycomb froth that is higher than 2 cm that persists for 10 minutes or longer.

Blood Agar Media (BAM): Is an agar cup semi-quantitative method that shows positive result of hemolytic halos. Role in plant ecology and impact on animal foraging.

Soybean isoflavones are valued in certain medicines, cosmetics, foods and feeds. Selection for high-isoflavone content in seeds along with agronomic traits is a goal of many soybean breeders.

The aim of the study was to identify the quantitative trait loci (QTL) underlying seed isoflavone content in soybean among seven environments in China. A cross was made between 'Zhongdou 27', a soybean Cited by: In this study, a HPLC methodology was established to determine quercetin which was a common hydrolyzate of the flalvonoid glycosides in the three plant medicines.

The determination method developed showed good linearity in the range of μg/mL, and had a Author: Ma Yue-ling, Chen Yu-jie, Wan Ding-rong, Chen ping, Xu Ran. The phenolic ingredients in samples of 24 cereal grains were analysed by HPLC, relying on the peak area of maximum absorption wavelength.

The chromatographic setup was comprised of a C18 column, a mobile phase with an elution gradient between solution A (acetic acid-water and methanol) and solution B (methanol and acetic acid-water solution.The progress in the last years in the interdisciplinary fields of chemistry, separation science, biology and pharmacy has boosted the natural product chemistry research [1–4], providing a valuable information about many classes of naturally occurring dietary these phytochemicals, polyphenols are the worldwide redox-active secondary metabolites of a phenolic nature [5–12].Cited by: 4.

Mapping expression quantitative trait loci (eQTL) of targeted genes represents a powerful and widely adopted approach to identify putative regulatory variants. Linking regulation differences to specific genes might assist in the identification of networks and interactions.

The objective of this study is to identify eQTL underlying expression of four gene families encoding isoflavone synthetic Cited by: